The overall objective of CAVICE project is Biodiversity assessment and monitoring.

Objective 1.

Investigation of functional diversity and metabolic activity of cave ice microbiome from SC and SV ice caves and VD and CH glacier caves.

       1.1. Sampling of ice sediments from SC, SV, VD, MY ice/glacier caves (2016 - 2017);

       1.2. Metagenome and metatranscriptom reconstitutution of all ice/glacier caves using NGS (2016 - 2018)

Objective 2. Survey of past and present climate variations impact on total and active microbiome from ice caves.

       2.1. Past climate stable isotopebased reconstruction in cave ice (2016 - 2017);

       2.2. Analysis of climate impact variations on cave ice microbiota (2017 - 2018).

Objective 3. Anthropogenic pollution impact on structural and functional diversity of cave ice

       3.1. Chemical analysis of cave ice cores from all ice/glacier caves (2016 - 2017);

       3.2. Identification of pollution biomarkers for the anthropogenic impact on cave ice (2017 - 2018).

Objective 4. Identification of novel psychrophilic/psychrotolerant strains of biotechnological potential.
       4.1. Screening for metal (Au/Ag/Fe) nanoparticle-producing strains from ice/glacier caves (2016 - 2018);

       4.2. Characterization and separation of biosynthesized metal nanoparticles (2017 - 2018)